Genetic analysis of human embryos by metaphase comparative genomic hybridization (mCGH) improves eff

Geoffrey Sher

Objective:To assess the benefit of selecting blastocysts for cryotransfer based upon prior comparative genomic hybridization (CGH) karyotyping of blastomeres derived from their cleaved embryos of origin. Implantation
and birth rates per transfer of previtrified CGH-tested blastocysts were compared with those following the transfer of nonCGH-tested fresh and warmed embryos.
Design:In vitro studies.
Setting:Private infertility clinic.
Patient(s):Women undergoing infertility treatment.
Intervention(s):Three groups of women with similar clinical and demographic characteristics were compared.Group A underwent transfer of warmed blastocysts derived from CGH-normal day 3 embryos. Group B underwent
embryo transfer of warmed blastocysts derived from nonkaryotyped vitrified embryos. Group C underwent fresh transfers with non-CGH-tested blastocysts.
Main Outcome Measure(s): Implantation and birth rates per embryo after the cryotransfer of CGH-tested blastocysts.

Result(s):

The birth rate per transfered blastocyst in group Awas 48%, versus 15% for group B and 19% for group C. The birth rate per embryo transfer was 60% for group A, and 33% for group B and 36% for group C. The miscarriage
rate was 4% in group A, 8% in group B, and 12% in group C.
Conclusion(s): The transfer of previously vitrified blastocysts derived from CGH-normal embryos significantly improves implantation and birth rates per embryo transfered and reduces the miscarriage rate. Vitrification does
not compromise this enhancement. (Fertil Steril_ 2009;92:1886–94._2009 by American Society for Reproductive Medicine.

Source: http://www.theifim.com/